An Open Sandwich Immunochromatography for Non-competitive Detection of Small Antigens
نویسندگان
چکیده
منابع مشابه
Protein-based open sandwich immuno-PCR for sensitive detection of small biomarkers.
Open sandwich (OS) immunoassay utilizes antigen-dependent stabilization of an antibody variable region to quantify various antigens, enabling noncompetitive detection of small molecules with a broad working range. For further improvement of its sensitivity, OS Immuno-PCR was attempted with recombinant fusion proteins. The maltose binding protein-fused heavy chain variable region (MBP-V(H)) of a...
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متن کامل[Sensitive noncompetitive measurement of small molecules by open sandwich immunoassay].
Proteins having multiple epitopes can be usually measured with sandwich ELISA employing two kinds of antibodies, which permits high sensitivity as well as a wide working range of more than three orders of magnitude. On the other hand, so-called monovalent antigens with MW less than 1000 are not susceptible to sandwich assays due to their small size and have almost always been measured in compet...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2021
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.20scp06